FO3X O3-FACE (Free-air O3 eXposure) is a multidisciplinary study to assess the effects of increasing tropospheric ozone and other stress factors on vegetation under open-air conditions. FO3X is an ecosystem-level manipulative research facility that ensures long-term operability, fully replicated treatment plots connected with state-of-the-art ecophysiology and genomics labs and is available for undertaking new collaborative projects. FO3X consists of nine 5x5x2m blocks in which the concentrations of tropospheric ozone and other stress factors (e.g., drought, nitrogen, pathogens) can be controlled. The design is a split-plot experiment that provides the ability to assess the effects of ozone and other stressors, alone and in combination, on many plant attributes, including growth, root characteristics, gas exchanges, biogenic volatile organic compounds, nutrients as well as antioxidants. At present, plants are potted but can be planted in the ground if needed.

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